Soil al., 1996; Torstenssen et al., 1998; Wyszkowska and

 Soil pollution with petroleum products is common
in areas where petroleum and natural gas are produced (Adam et al., 2002;
Clark, 2003). The production  of  as well as the use of petroleum products  lead to pollution of soil (Ayotamuno et al.,
2006).Moreover, accidental spills caused by pipeline leakages and ruptures have
been reported (Ogbo, 2009).  Soil pollution
alters soil physical and chemical characteristics, which reduced its fertility and
influence plant production (Gong et al., 1996; Torstenssen et al., 1998;
Wyszkowska and Kucharski, 2000; Wyszkowski et al., 2004; Wyszkowski and
Wyszkowska, 2005).

             
Diesel is a mixture of hydrocarbons that is consists of alkanes,
cycloalkanes and aromatic compounds (Frankenberger and Jahanson, 1982; Speight,
1992, 2007). It also contains sulfur, nitrogen and oxygen in low concentrations
as well as metals (Posthuma, 1970).

We Will Write a Custom Essay Specifically
For You For Only $13.90/page!


order now

Petroleum pollution of soil
affects soil biochemical properties (Achuba and Okoh, 2014, Chikezie et al.,
2016), which in turn affect the growth and metabolic activities of exposed
plants (Achuba2006; Achuba and Okoh 2015; Achuba and Asagba, 2016. Previous
studies reported reduced germination of seeds in soil contaminated by petroleum
products (Amakiri and Onofeghara, 1984; Adam and Duncan, 1999, 2002; Vavrek and
Campbell, 2002; Achuba, 2006; Smith et al., 2006, Sharifi et al., 2007; Korade
and Fulekar, 2009; Ogbo, 2009).  Contamination of the soil with diesel oil
limits the available essential soil mineral needed by plant (Roy et al, 2013)
and  overall  plant growth  (Nwaogu et al, 2006; Das and Chandran, 2011).

Previous
researches have been carried out on different organic wastes. Some of these
studies include the investigation on the effect of nutrient amendments of
diesel oil polluted soil on growth parameters of eggplant (Solanum melongena)
that involved treatment of polluted with different amounts of organic and
inorganic fertilizers (Akujobi et al, 2011) and also the effects of abattoir
effluent treatment of diesel contaminated soil on plant metabolic status in
addition to microbial degradation of diesel  in soil were evaluated (Umanu and Owoseni,
2013; Achuba and Erihijivwo,
2017 ). The application of pond wastewater as a
fertilizer and soil conditioner has not been widely reported.

Main purpose

 The application of pond waste water could be a
very attractive proposal. This study was conducted to investigate the effect of
pond waste water on the growth and metabolism of cowpea seedlings grown in
diesel contaminated soil.

 

Significant of Study

This research
attempts to exploit pond waste water as an organic fertilizer actively involved
in the remediation of diesel polluted soil. Local farmers with minimal or no
access to inorganic fertilizer in the Niger Delta area of Nigeria could benefit
from this research due to the fact that pond waste water is readily available
and is a cheap source of organic fertilizer. Also, the issue of proper ways of
disposing this pond waste water could be cocktailed with this research.

  Scope of Study

This
study investigated the effect of pond waste water on both biomolecules and metabolism
parameters of cowpea seedlings grown in diesel contaminated soil. The
biomolecules include protein, glucose, amino acid, total sugar, total
chlorophyll, and  beta carotene while the
biochemical parameters determined include lipid peroxidation, superoxide dismutase
activity, catalase activity, xanthine oxidase activity, aldehyde oxidase
activity, ? amylase activity, and phosphorylase activity.

1.4   Aims and
Objectives

The
aim of the study was conducted to investigate the effect of artificial pond
waste water on the growth and metabolism of cowpea grown in a diesel
contaminated soil.

The
specific objectives of this study are to highlight:

i The effect of artificial
pond waste water on diesel induced alterations of the metabolism of
macromolecules in cowpea seedlings.

ii The effect of
artificial pond waste water on diesel mediated oxidative stress in cowpea
seedlings.

iii The effect
of artificial pond waste water on drug metabolizing enzymes in cowpea seedlings

x

Hi!
I'm Neil!

Would you like to get a custom essay? How about receiving a customized one?

Check it out